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Chicken GHRL (Ghrelin) ELISA Kit

Chicken GHRL (Ghrelin) ELISA Kit

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$588.00 USD
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Catalogue Number: ELK4502

Alternative Names: MTLRP; Growth Hormone-Releasing Peptide

Species: Chicken

Assay Type: Competitive Inhibition

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research: Signal transduction;Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ghrelin(GHRL) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ghrelin(GHRL). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ghrelin(GHRL) in the samples is then determined by comparing the OD of the samples to the standard curve.