{"product_id":"chip-monoclonal-antibody-mb67252","title":"CHIP Monoclonal Antibody-MB67252","description":"\u003cp\u003eCHIP Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB67252-50, MB67252-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9UNE7\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/1000 - 1\/2000)\u003c\/p\u003e\n\n\u003cp\u003eBackground: The carboxy terminus of Hsc70-interacting protein (CHIP, STUB1) is a co-chaperone protein and functional E3 ubiquitin ligase that links the polypeptide binding activity of Hsp70 to the ubiquitin proteasome system. Cytoplasmic CHIP protein contains three 34-amino acid TPR (tetratricopeptide repeat) domains at its amino terminus and a carboxy-terminal U-box domain. CHIP interacts with the molecular chaperones Hsc70-Hsp70 and Hsp90 through its TPR domain, while E3 ubiquitin ligase activity is confined to the U-box domain. The binding of CHIP to Hsp70 can stall the folding of Hsp70 client proteins and concomitantly facilitate the U-box dependent ubiquitination of Hsp70-bound substrates. CHIP appears to play a central role in cell stress protection and is responsible for the degradation of disease-related proteins that include cystic fibrosis transmembrane conductance regulator, p53, huntingtin and Ataxin-3, Tau protein, and α-synuclein.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: This antibody is purified through a protein G column.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of CHIP protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of CHIP expression in 293 (A), Hela (B), HepG2 (C), L929 (D) whole cell lysates.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 32 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CHIP; E3 ubiquitin-protein ligase CHIP; Antigen NY-CO-7; CLL-associated antigen KW-8; Carboxy terminus of Hsp70-interacting protein; STIP1 homology and U box-containing protein 1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human CHIP. The exact sequence is proprietary.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42906420674739,"sku":"MB67252-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42906420707507,"sku":"MB67252-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_1548af29-b59b-4e95-b63f-f6a44b3a63b8.jpg?v=1702919284","url":"https:\/\/afsbio.com\/products\/chip-monoclonal-antibody-mb67252","provider":"AFSBio Inc.","version":"1.0","type":"link"}