Cholesterol oxidase is a flavoprotein involved in the first step of cholesterol catabolism. The enzyme first oxidizes cholesterol to cholest-5-en-3-one then isomerizes it to cholest-4-en-3-one while producing hydrogen peroxide H2O2. Available in a 250 Units and custom bulk sizes.
| Product Data Sheet | Download PDF |
| MSDS | Download PDF |
| Catalog # | P2010002 |
| Size | 250 U |
| Molecular Weight | 34 kDa |
| Reaction catalyzed | Cholesterol + O₂ –> Cholest-4-en-3-one + H₂O₂ |
| Unit Definition | One enzymatic unit of cholesterol oxidase converts 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. |
| Specific Activity | 21 U/mg |
| Storage | -20°C. Avoid repeated freeze/thaw cycles. |
| Supplied as | Yellow lyophilized powder (11.9 mg). |
| Application | Assaying cholesterol |
| Keywords | Cholesterol oxidase, cholesterol, flavoprotein. |
References
- Kreit, J., Sampson, N. S. (2009). Cholesterol oxidase: physiological functions. FEBS Journal, 276(23), 6844–6856.
- Vrielink, A., and Ghisla, S. (2009). Cholesterol oxidase: biochemistry and structural features. FEBS Journal, 276(23), 6826–6843.
- Vrielink, A. (2010). Cholesterol Oxidase: Structure and Function. Cholesterol Binding and Cholesterol Transport Proteins: Subcellular Biochemistry, 137–158.
- Niwas, R., Singh, V., Singh, R., Pant, G., Mitra, K., and Tripathi, C. K. M. (2014). Cholesterol oxidase production from entrapped cells ofStreptomycessp. Journal of Basic Microbiology, 54(11), 1233–1239.
- Pollegioni, L. (2009). Cholesterol oxidase: a model flavoprotein oxidase and a biotechnological tool. FEBS Journal, 276(23), 6825–6825.