Cholesterol Oxidase (250 U)

Cholesterol Oxidase (250 U)

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Cholesterol oxidase is a flavoprotein involved in the first step of cholesterol catabolism. The enzyme first oxidizes cholesterol to cholest-5-en-3-one then isomerizes it to cholest-4-en-3-one while producing hydrogen peroxide H2O2. Available in a 250 Units and custom bulk sizes.


Product Data Sheet Download PDF
MSDS Download PDF
Catalog # P2010002
Size 250 U
Molecular Weight 34 kDa
Reaction catalyzed Cholesterol + O₂ –> Cholest-4-en-3-one + H₂O₂
Unit Definition One enzymatic unit of cholesterol oxidase converts 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C.
Specific Activity 21 U/mg
Storage -20°C. Avoid repeated freeze/thaw cycles.
Supplied as Yellow lyophilized powder (11.9 mg).
Application Assaying cholesterol
Keywords Cholesterol oxidase, cholesterol, flavoprotein.



  1. Kreit, J., Sampson, N. S. (2009). Cholesterol oxidase: physiological functions. FEBS Journal, 276(23), 6844–6856.
  2. Vrielink, A., and Ghisla, S. (2009). Cholesterol oxidase: biochemistry and structural features. FEBS Journal, 276(23), 6826–6843.
  3. Vrielink, A. (2010). Cholesterol Oxidase: Structure and Function. Cholesterol Binding and Cholesterol Transport Proteins: Subcellular Biochemistry, 137–158.
  4. Niwas, R., Singh, V., Singh, R., Pant, G., Mitra, K., and Tripathi, C. K. M. (2014). Cholesterol oxidase production from entrapped cells ofStreptomycessp. Journal of Basic Microbiology, 54(11), 1233–1239.
  5. Pollegioni, L. (2009). Cholesterol oxidase: a model flavoprotein oxidase and a biotechnological tool. FEBS Journal, 276(23), 6825–6825.