{"product_id":"ckb-polyclonal-antibody-bs90306","title":"CKB Polyclonal Antibody-BS90306","description":"\u003cp\u003eCKB Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90306-50, BS90306-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P12277(Human) Q04447(Mouse) P07335(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC, IF, IHC, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500-1:2,000\u003cbr\u003e\nICC:1:50-1:200\u003cbr\u003e\nIHC:1:50-1:200\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Creatine kinases (CKs) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. Creatine kinases provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems including muscle, electrocytes, retina photoreceptor cells, brain cells, kidney, salt glands, myometrium, placenta, pancreas, thymus, thyroid, intestinal epithelial cells, endothelial cells, cartilage and bone cells, macrophages, blood platelets, and tumor and cancer cells. Human cytoplasmic creatine kinase-B, also designated CK-B and BCK, is a 381 amino acid, brain tissue specific isoform of creatine kinase. Human cytoplasmic creatine kinase-muscle (CK-M, MCK) is a muscle tissue-specific isoform of creatine kinase. Human cytoplasmic creatine kinase-Mi (Mi-CK, MtCK) is a 416 amino acid mitochondrial-specific isoform of creatine kinase. Cytosolic creatine kinases are important in the energetic regulation of Ca2+-pumps and in the maintenance of Ca2+-homeostasis.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: CKB polyclonal antibody detects endogenous levels of CKB protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 43 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Creatine kinase B type on different lysates using anti-Creatine kinase B type antibody at 1\/500 dilution.\u003cbr\u003e\n Positive control:\u003cbr\u003e\n Lane 1: Mouse brain \u003cbr\u003e\n Lane 2: Mouse brain\u003cbr\u003e\n Lane 3: Rat brain \u003cbr\u003e\n Lane 4: 293, ICC staining Creatine kinase B type in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: B-CK, CPK-B, CKB, CKBB, B CK, B-CK, BB-CK, BCK, Brain creatine kinase, Ckb, CKBB, Creatine kinase B, Creatine kinase B chain, Creatine kinase B type, Creatine kinase B-type, Creatine Kinase BB Isoenzyme, Creatine kinase brain, Creatine kinase brain type, Creatine phosphokinase BB, Epididymis luminal protein 211, Epididymis secretory protein Li 29, HEL 211, HEL S 29, KCRB,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979800055987,"sku":"BS90306-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979800088755,"sku":"BS90306-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_3b292e2b-0fff-442d-b9cc-eb19c88ad82a.jpg?v=1705752789","url":"https:\/\/afsbio.com\/products\/ckb-polyclonal-antibody-bs90306","provider":"AFSBio Inc.","version":"1.0","type":"link"}