{"product_id":"cleaved-caspase-4-5-p20-d270-d311-rabbit-polyclonal-antibody-es7702","title":"Cleaved-Caspase-4\/5 p20 (D270\/D311) Rabbit Polyclonal Antibody - ES7702","description":"\u003cp\u003eCleaved-Caspase-4\/5 p20 (D270\/D311) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES7702-50, ES7702-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: CASP4, ICH2, Caspase-4, CASP-4, ICE(rel)-II, Protease ICH-2, Protease TX, CASP5, ICH3, Caspase-5, CASP-5, ICE(rel)-III, Protease ICH-3, Protease TY\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human Caspase 4\/5. AA range:221-270\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: Caspase-4\/5\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Cleaved-Caspase-4\/5 p20 (D270\/D311) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-4\/5 p20 protein resulting from cleavage adjacent to D270\/D311.\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/500 - 1\/2000. ELISA: 1\/40000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e\n\n\u003cp\u003eFormlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level), Not detected in brain,\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 47,22kD\u003c\/p\u003e\n\n\u003cp\u003eFields: Neutrophil extracellular trap formation, NOD-like receptor signaling pathway, Pathogenic Escherichia coli infection, Shigellosis, Salmonella infection\u003c\/p\u003e\n\n\u003cp\u003eFunction: Catalytic Activity: Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at Asp-Glu-Val-Asp-|-\u003cbr\u003e\nFunction: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1\u003cbr\u003e\nPTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8\u003cbr\u003e\nSimilarity: Belongs to the peptidase C14A family\u003cbr\u003e\nSimilarity: Contains 1 CARD domain\u003cbr\u003e\nSubunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit\u003cbr\u003e\nTissue Specificity: Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain\u003c\/p\u003e\n\n\n\u003cp\u003eSubcellular Location: Cytoplasm, cytosol Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side Mitochondrion Inflammasome Secreted Predominantly localizes to the endoplasmic reticulum (ER) Association with the ER membrane requires TMEM214 (PubMed:15123740) Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630)\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: CASP4\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: Caspase4\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":46313740730547,"sku":"ES7702-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":46313740763315,"sku":"ES7702-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk_antibodies_d75295e2-ffc1-4e0f-a3e7-87715c8983a9.jpg?v=1793190507","url":"https:\/\/afsbio.com\/products\/cleaved-caspase-4-5-p20-d270-d311-rabbit-polyclonal-antibody-es7702","provider":"AFSBio Inc.","version":"1.0","type":"link"}