{"product_id":"clia-kit-for-anterior-gradient-2-agr2-scc285hu","title":"CLIA Kit for Anterior Gradient 2 (AGR2) - SCC285Hu","description":"\u003cp\u003eCLIA Kit for Anterior Gradient 2 (AGR2)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 24T, 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: SCC285Hu-24, SCC285Hu-48, SCC285Hu-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eFeatured Series: Sandwich kit\u003c\/p\u003e\n\n\u003cp\u003eTarget: Anterior Gradient 2\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: GOB4; HAG2; XAG2; AG2; PDIA17; HPC8; Anterior Gradient Protein 2; Protein Disulfide Isomerase Family A, Member 17; Secreted cement gland protein XAG-2 homolog\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eUniprot: O95994\u003c\/p\u003e\n\n\u003cp\u003eGene ID: 10551\u003c\/p\u003e\n\n\u003cp\u003eFeatured Series Function: Detects protein (regular version)\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Reactive with Human AGR2 \/ Anterior Gradient 2\u003c\/p\u003e\n\n\u003cp\u003eMethod: Chemiluminescence\u003c\/p\u003e\n\n\u003cp\u003eDetection principle: Double-antibody Sandwich\u003c\/p\u003e\n\n\u003cp\u003eDetection\u003cbr\u003e\nrange: 13.7-10,000pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 5.5pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 2h, 40min\u003c\/p\u003e\n\n\u003cp\u003eSample Size: 100uL\u003c\/p\u003e\n\n\u003cp\u003eRecommended\/Predicted\u003cbr\u003e\nSample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\n\n\u003cp\u003eStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\n\n\u003cp\u003eShelf-life: 12 months\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This assay has high sensitivity and excellent specificity for detection of Anterior Gradient 2 (AGR2).\u003cbr\u003e\nNo significant cross-reactivity or interference between Anterior Gradient 2 (AGR2) and analogues was observed.\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anterior Gradient 2 (AGR2) were tested 20 times on one plate, respectively.\u003cbr\u003e\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anterior Gradient 2 (AGR2) were tested on 3 different plates, 8 replicates in each plate.\u003cbr\u003e\nCV(%) = SD\/meanX100\u003c\/p\u003e\n\n\u003cp\u003eStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\u003cbr\u003e\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\n\n\u003cp\u003eTarget: 1. Prepare all reagents, samples and standards;\u003cbr\u003e\n2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;\u003cbr\u003e\n3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;\u003cbr\u003e\n4. Aspirate and wash 3 times;\u003cbr\u003e\n5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\u003cbr\u003e\n6. Aspirate and wash 5 times;\u003cbr\u003e\n7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;\u003cbr\u003e\n8. Read RLU value immediately.\u003c\/p\u003e\n\n\u003cp\u003eTest principle: The microplate provided in this kit has been pre-coated with an antibody specific to Anterior Gradient 2 (AGR2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Anterior Gradient 2 (AGR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Anterior Gradient 2 (AGR2) level in the sample or standard.;\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Tumor immunity; Reproductive science; Bone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Cloud-Clone","offers":[{"title":"24T","offer_id":45379969777843,"sku":"SCC285Hu-24","price":470.4,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":45379969810611,"sku":"SCC285Hu-48","price":823.2,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45379969843379,"sku":"SCC285Hu-96","price":1176.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Cloud-Clone.jpg?v=1774117629","url":"https:\/\/afsbio.com\/products\/clia-kit-for-anterior-gradient-2-agr2-scc285hu","provider":"AFSBio Inc.","version":"1.0","type":"link"}