{"product_id":"clia-kit-for-parathyroid-hormone-pth-ucca866ra","title":"CLIA Kit for Parathyroid Hormone (PTH) - UCCA866Ra","description":"\u003cp\u003eCLIA Kit for Parathyroid Hormone (PTH)\u003c\/p\u003e\n\n\u003cp\u003eSize: 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: UCCA866Ra-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eTarget: Parathyroid Hormone (PTH)\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Rat\u003c\/p\u003e\n\n\u003cp\u003eDetection range: 7.81-2,000pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 2.65pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eUniprot: P04089\u003c\/p\u003e\n\n\u003cp\u003eGene ID: 24694\u003c\/p\u003e\n\n\u003cp\u003eFeatured Series Function: Detects small molecule\u003c\/p\u003e\n\n\u003cp\u003eRecommended\/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 2h\u003c\/p\u003e\n\n\u003cp\u003eMethod: Chemiluminescence\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Reactive with Rat PTH \/ Parathyroid Hormone\u003c\/p\u003e\n\n\u003cp\u003eDetection principle: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSample Size: 50uL\u003c\/p\u003e\n\n\u003cp\u003eAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\n\n\u003cp\u003eStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\n\n\u003cp\u003eShelf-life: 12 months\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This assay has high sensitivity and excellent specificity for detection of Parathyroid Hormone (PTH).\u003cbr\u003e\nNo significant cross-reactivity or interference between Parathyroid Hormone (PTH) and analogues was observed.\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested 20 times on one plate, respectively.\u003cbr\u003e\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested on 3 different plates, 8 replicates in each plate.\u003cbr\u003e\nCV(%) = SD\/meanX100\u003c\/p\u003e\n\n\u003cp\u003eStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\u003cbr\u003e\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\n\n\u003cp\u003eAssay procedure summary: 1. Prepare all reagents, samples and standards;\u003cbr\u003e\n2. Add 50µL standard or sample to each well.\u003cbr\u003e\n And then add 50µL prepared Detection Reagent A immediately.\u003cbr\u003e\n Shake and mix. Incubate 1 hour at 37°C;\u003cbr\u003e\n3. Aspirate and wash 3 times;\u003cbr\u003e\n4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\u003cbr\u003e\n5. Aspirate and wash 5 times;\u003cbr\u003e\n6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;\u003cbr\u003e\n7. Read RLU value immediately.\u003c\/p\u003e\n\n\u003cp\u003eTest principle: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Parathyroid Hormone (PTH). A competitive inhibition reaction is launched between biotin labeled Parathyroid Hormone (PTH) and unlabeled Parathyroid Hormone (PTH) (Standards or samples) with the pre-coated antibody specific to Parathyroid Hormone (PTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Parathyroid Hormone (PTH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Parathyroid Hormone (PTH) level in the sample or standard.\u003c\/p\u003e \n\n\u003cp\u003eResearch Area: Metabolic pathway; Endocrinology; Hormone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"USCN","offers":[{"title":"Default Title","offer_id":45390098137267,"sku":"UCCA866Ra-96","price":638.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/USCN.png?v=1774797733","url":"https:\/\/afsbio.com\/products\/clia-kit-for-parathyroid-hormone-pth-ucca866ra","provider":"AFSBio Inc.","version":"1.0","type":"link"}