{"product_id":"cncbl-cyanocobalamin-elisa-kit-elk7871","title":"CNCbl (Cyanocobalamin) ELISA Kit - ELK7871","description":"\u003cp\u003eCNCbl (Cyanocobalamin) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK7871-48, ELK7871-96, ELK7871-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: VB12; Vitamin B12; Cobalamin; α-5,6-Dimethylbenzimidazolyl Cobamidcyanide\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 49.1 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 156.25-10000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Metabolic pathway; Nutrition metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CNCbl. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CNCbl. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CNCbl in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212217876659,"sku":"ELK7871-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212217909427,"sku":"ELK7871-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200779346099,"sku":"ELK7871-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_5ee008d6-8c86-4e1f-9f28-4d6d1a54b64f.png?v=1767063721","url":"https:\/\/afsbio.com\/products\/cncbl-cyanocobalamin-elisa-kit-elk7871","provider":"AFSBio Inc.","version":"1.0","type":"link"}