{"product_id":"cot-p284-polyclonal-antibody-bs1074","title":"Cot (P284) Polyclonal Antibody-BS1074","description":"\u003cp\u003eCot (P284) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1074-50, BS1074-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P41279\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eBackground: The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine\/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH\/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1.\u003c\/p\u003e \n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: COT (P284) polyclonal antibody detects endogenous levels of COT protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Cot (P284) pAb at 1:500 dilution\u003cbr\u003e\nLane 1: L02 whole cell lysate (40ug)\u003cbr\u003e\nLane 2: SGC7901 whole cell lysate (40ug)\u003cbr\u003e\nLane 3: PC3 whole cell lysate (40ug)\u003cbr\u003e\nLane 4: H9C2 whole cell lysate (40ug)\u003cbr\u003e\nLane 5: AML12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of COT (P284) pAb in paraffin-embedded human brain tissue.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 53 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine\/threonine-protein kinase cot; Tumor progression locus 2; TPL-2; MAP3K8; COT; ESTF\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 250-300 of Human Cot.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953206104243,"sku":"BS1074-50","price":254.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953206137011,"sku":"BS1074-100","price":442.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_f0bfeca9-ed7f-4f74-b5bc-89afd32fe644.jpg?v=1705056746","url":"https:\/\/afsbio.com\/products\/cot-p284-polyclonal-antibody-bs1074","provider":"AFSBio Inc.","version":"1.0","type":"link"}