{"product_id":"cot-phospho-t290-polyclonal-antibody-bs4051","title":"Cot (phospho-T290) Polyclonal Antibody-BS4051","description":"\u003cp\u003eCot (phospho-T290) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS4051-50, BS4051-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P41279\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eBackground: The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine\/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH\/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1.\u003c\/p\u003e \n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: p-Cot (T290) polyclonal antibody detects endogenous levels of Cot protein only when phosphorylated at Thr290.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Immunohistochemistry (IHC) analyzes of p-Cot (T290) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 53 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine\/threonine-protein kinase cot; Tumor progression locus 2; TPL-2; MAP3K8; COT; ESTF\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human Cot around the phosphorylation site of Threonine 290.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42949474746547,"sku":"BS4051-50","price":270.48,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42949474779315,"sku":"BS4051-100","price":479.78,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_5d5ac435-8e99-4b8a-9926-799198043a1a.jpg?v=1704970204","url":"https:\/\/afsbio.com\/products\/cot-phospho-t290-polyclonal-antibody-bs4051","provider":"AFSBio Inc.","version":"1.0","type":"link"}