{"product_id":"creb-1-phospho-ser133-rabbit-polyclonal-antibody-es1292","title":"CREB-1 (phospho Ser133) Rabbit Polyclonal Antibody - ES1292","description":"\u003cp\u003eCREB-1 (phospho Ser133) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES1292-50, ES1292-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: approximately 7-10 business days, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016],\u003c\/p\u003e \n\n\u003cp\u003eReactivity: Human, Mouse, Rat, Pig\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: IF, WB, IHC, IP, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser133. AA range:100-149\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: CREB-1,\u003c\/p\u003e \n\n\u003cp\u003eSpecificity: Phospho-CREB-1 (S133) Polyclonal Antibody detects endogenous levels of CREB-1 protein only when phosphorylated at S133.\u003c\/p\u003e\n\n\u003cp\u003eDilution: IF: 1:50-200 Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. Immunoprecipitation: 2-5 ug\/mg lysate. ELISA: 1\/10000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e \n\n\u003cp\u003eFormulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Eye, Placenta, Spleen, Testis,\u003c\/p\u003e \n\n\u003cp\u003eObserved Band (KD): 37kD\u003c\/p\u003e\n\n\u003cp\u003eFields: cGMP-PKG signaling pathway, cAMP signaling pathway, PI3K-Akt signaling pathway, AMPK signaling pathway, Longevity regulating pathway, Adrenergic signaling in cardiomyocytes, Osteoclast differentiation, Antigen processing and presentation, TNF signaling pathway, Circadian rhythm, Circadian entrainment, Thermogenesis, Cholinergic synapse, Dopaminergic synapse, Insulin secretion, Estrogen signaling pathway, Melanogenesis, Thyroid hormone synthesis, Glucagon signaling pathway, Renin secretion, Aldosterone synthesis and secretion, Relaxin signaling pathway, Cortisol synthesis and secretion, Parathyroid hormone synthesis, secretion and action, Insulin resistance, Cushing syndrome, Growth hormone synthesis, secretion and action, Vasopressin-regulated water reabsorption, Huntington disease, Prion disease, Cocaine addiction, Amphetamine addiction, Alcoholism, Tuberculosis, Hepatitis B, Human cytomegalovirus infection, Human papillomavirus infection, Human\u003c\/p\u003e\n\n\u003cp\u003eFunction: Disease: A chromosomal aberration involving CREB1 is associated with angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1\/CREB1 fusion gene that is most common genetic abnormality in this tumor type. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transciption activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity. PTM: Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. similarit\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Nucleus\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: CREB1\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: Cyclic AMP-responsive element-binding protein 1\u003c\/p\u003e\n\n\u003cp\u003eHuman Gene ID: 1385\u003c\/p\u003e\n\n\u003cp\u003eHuman SwissProt Number: P16220\u003c\/p\u003e\n\n\u003cp\u003eMouse Gene ID: 12912\u003c\/p\u003e\n\n\u003cp\u003eMouse SwissProt Number: Q01147\u003c\/p\u003e\n\n\u003cp\u003eRat Gene ID: 81646\u003c\/p\u003e\n\n\u003cp\u003eRat SwissProt Number: P15337\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":45825501986995,"sku":"ES1292-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45825502019763,"sku":"ES1292-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk-biotechnology-logo-product.jpg?v=1786476023","url":"https:\/\/afsbio.com\/products\/creb-1-phospho-ser133-rabbit-polyclonal-antibody-es1292","provider":"AFSBio Inc.","version":"1.0","type":"link"}