{"product_id":"creb-rabbit-monoclonal-antibody-bs9811m","title":"CREB Rabbit Monoclonal Antibody-BS9811M","description":"\u003cp\u003eCREB Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS9811M-50, BS9811M-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P16220\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat, Zebra fish\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF, IHC, IP, , FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000-1:5000 IHC\/ICC\/IF: 1:50-1:200 \u003cbr\u003e\nIP: 1:50-1:200 FC: 1:10-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cyclic AMP Response Element Binding protein (CREB) is a basic \/ leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 \/ 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of CREB and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 38 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of CREB Rabbit mAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: Hela whole cell lysate\u003cbr\u003e\n Lane 2: HepG2 whole cell lysate\u003cbr\u003e\n Lane 3: Jurkat whole cell lysate\u003cbr\u003e\n Lane 4: NIH\/3T3 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CREB antibody. Counter stained with hematoxylin., ICC staining CREB in Hela cells (green). The nuclear counter stain is DAPI (blue). \u003cbr\u003e\n Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1; CREB1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant antibody.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43004763832499,"sku":"BS9811M-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43004763865267,"sku":"BS9811M-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e886e4d1-0b7b-49a5-8ebe-2bf9300d8f60.jpg?v=1706445951","url":"https:\/\/afsbio.com\/products\/creb-rabbit-monoclonal-antibody-bs9811m","provider":"AFSBio Inc.","version":"1.0","type":"link"}