{"product_id":"crispr-cas9-mouse-monoclonal-antibody-mb9030","title":"CRISPR-Cas9 Mouse Monoclonal Antibody-MB9030","description":"\u003cp\u003eCRISPR-Cas9 Mouse Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB9030-50, MB9030-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgM, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q99ZW2\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Recombinant Cas9\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000-5000\u003cbr\u003e\nICC: 1:50-200\u003c\/p\u003e\n\n\u003cp\u003eBackground: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9\/crRNA\/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects recombinant Cas9 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 158 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of CRISPR\/Cas9 Mouse mAb at 1:5000 dilution\u003cbr\u003e\n Lane 1: CRISPR\/Cas9 transfected 293 cells lysate \u003cbr\u003e\n Lane 2: Non-transfected 293 cells lysate, Immunofluorescence staining CRISPR\/Cas9 in CRISPR\/Cas9 transfected 293T cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Cas9; CRISPR-associated endonuclease Cas9\/Csn1; CRISPR-Cas9\/Csn1; csn1; SpyCas9\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: This antibody is produced by immunizing mice with a recombinant protein corresponding to a sequence of CRISPR\/Cas9.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989567672499,"sku":"MB9030-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989567705267,"sku":"MB9030-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_7e279c3e-0fb3-4439-8738-8bf6bd88d407.jpg?v=1706095511","url":"https:\/\/afsbio.com\/products\/crispr-cas9-mouse-monoclonal-antibody-mb9030","provider":"AFSBio Inc.","version":"1.0","type":"link"}