{"product_id":"crispr-cas9-sp-polyclonal-antibody-bs90331","title":"CRISPR-Cas9 SP Polyclonal Antibody-BS90331","description":"\u003cp\u003eCRISPR-Cas9 SP Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90331-50, BS90331-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q99ZW2\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Recombinant Cas9\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9\/crRNA\/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of CRISPR\/Cas9 (R780) polyclonal antibody at 1:500 dilution\u003cbr\u003e\n Lane 1: HEK293T whole cell lysate. \u003cbr\u003e\n Lane 2: HEK293T transfected with CRISPR\/Cas9 (L:P=3:1). \u003cbr\u003e\n Lane 3: HEK293T transfected with CRISPR\/Cas9 (L:P=6:1). \u003cbr\u003e\n Lane 4: HEK293T transfected with CRISPR\/Cas9 (L:P=9:1).\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 160 kDa\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: CRISPR-Cas9 SP polyclonal antibody detects endogenous levels of CRISPR-Cas9 SP protein.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Cas9; CRISPR-associated endonuclease Cas9\/Csn1; CRISPR-Cas9\/Csn1; csn1; SpyCas9\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 750-800 of CRISPR\/Cas9.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42939196211379,"sku":"BS90331-50","price":415.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42939196244147,"sku":"BS90331-100","price":640.78,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_3d3381e1-ba8b-4f7d-b7eb-54eec97bc4e9.jpg?v=1704544883","url":"https:\/\/afsbio.com\/products\/crispr-cas9-sp-polyclonal-antibody-bs90331","provider":"AFSBio Inc.","version":"1.0","type":"link"}