{"product_id":"crk-monoclonal-antibody-mb66546","title":"CRK Monoclonal Antibody - MB66546","description":"\u003cp\u003eCRK Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66546-50, MB66546-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: CrkII, a cellμlar homologue of v-Crk, belongs to a family of adaptor proteins with an SH2-SH3-SH3 domain structure that transmits signals from tyrosine kinases. The primary function of Crk is to recruit cytoplasmic proteins in the vicinity of tyrosine kinases through SH2-phospho-tyrosine interaction. Thus, the output from Crk depends on the SH3-binding proteins, which include the C3G and Sos guanine nucleotide exchange proteins, Abl tyrosine kinase, DOCK180 and some STE20-related kinases. The variety of Crk-binding proteins indicates the pleiotropic function of Crk. The two CrkII SH3 domains are separated by a 54 amino acid linker region, which is highly conserved in Xenopus, chicken and mammalian CrkII proteins. Tyrosine 221 in this region is phosphorylated by the Abl tyrosine kinase, IGF-I receptorand EGF receptor. Once Tyr221 is phosphorylated, CrkII undergoes a change in intramolecμlar folding and SH2-pTyr interaction, which causes rapid dissociation of CrkII from the tyrosine kinase complex.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Adapter molecμle crk, Proto-oncogene c-Crk, p38\u003c\/p\u003e\n\n\u003cp\u003eClonality: Monoclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Purified recombinant human CrkII protein fragments expressed in E.coli.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB (1\/500 - 1\/1000)\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of CRK protein.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 42 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: Regμlation of Actin Dynamics，MAPK Erk in Growth and Differentiation Pathway(，Death Receptor Signaling，SAPK JNK Signaling Cascades\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: P46108\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45946409025715,"sku":"MB66546-50","price":274.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946409058483,"sku":"MB66546-100","price":457.5,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/crk-monoclonal-antibody-mb66546","provider":"AFSBio Inc.","version":"1.0","type":"link"}