{"product_id":"crkl-phospho-y207-polyclonal-antibody-bs4056","title":"CrkL (phospho-Y207) Polyclonal Antibody-BS4056","description":"\u003cp\u003eCrkL (phospho-Y207) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS4056-50, BS4056-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P46109\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eCrkL (phospho-Y207) Polyclonal Antibody-BS4056\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Crk, is a transformation-specific protein that induces elevation of cellular phosphotyrosine levels, but lacks tyrosine kinase activity itself. A second protein, Nck, consists solely of three SH3 domains and one SH2 domain, while GRB2 contains an SH2 domain flanked on both sides by SH3 domains. A member of this protein class, Crk-L, is encoded by a gene located on chromosome 22, band 11, centromeric of the chronic myelogenous leukemia breakpoint region. Crk-L encodes a 303 amino acid protein with one SH2 and two SH3 domains, and a predicted molecular weight of 36 kDa.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: p-Crk-L (Y207) polyclonal antibody detects endogenous levels of Crk-L protein only when phosphorylated at Tyr207.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 39 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of p-CrkL (Y207) pAb at 1:500 dilution\u003cbr\u003e\n Lane 1: MCF-7 whole cell lysate (40ug) \u003cbr\u003e\n Lane 2: HEK293T whole cell lysate (40ug) \u003cbr\u003e\n Lane 3: PMVEC whole cell lysate (40ug) \u003cbr\u003e\n Lane 4: CT26 whole cell lysate (40ug) \u003cbr\u003e\n Lane 5: SGC7901 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-Crk-L (Y207) pAb in paraffin-embedded human colon cancer tissue.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Crk L; Crk like protein; Crk-like protein; Crkl; Crkol; Oncogene CrkL;\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human CrkL around the phosphorylation site of Tyrosine 207.\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957295386803,"sku":"BS4056-50","price":276.71,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957295419571,"sku":"BS4056-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e9366f09-d387-4db3-bd75-134956eb1c3f.jpg?v=1705144489","url":"https:\/\/afsbio.com\/products\/crkl-phospho-y207-polyclonal-antibody-bs4056","provider":"AFSBio Inc.","version":"1.0","type":"link"}