{"product_id":"cstf-50-r3-polyclonal-antibody-bs2036","title":"CstF-50 (R3) Polyclonal Antibody-BS2036","description":"\u003cp\u003eCstF-50 (R3) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers:BS2036-50, BS2036-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q05048\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: IHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits of 77, 64 and 50 kDa. Heterotrimeric CstF recognizes GU and U-rich sequences located downstream of the polyadenylation site on RNA. The 50 kDa CstF subunit shares extensive homology with mammalian G protein beta-subunits and has a transducin repeat domain, which is a 44 amino acid-long sequence that is repeated seven times. CstF-50 interacts with the nuclear protein BARD1 (BRCA1-associated RING domain protein) and inhibits polyadenylation in vitro. CstF-50 may also be responsible for the interaction of the heterotrimeric CstF complex with other polyadenylation and 3'-end cleavage factors to form a stable complex on the pre-mRNA.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 50 kDa\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: CstF-50 (R3) polyclonal antibody detects endogenous levels of CstF-50 protein\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003cbr\u003e\nAlternative Name: Cleavage stimulation factor subunit 1; CF-1 50 kDa subunit; Cleavage stimulation factor 50 kDa subunit; CSTF 50 kDa subunit; CstF-50; CstF50; CSTF1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to the N-terminus of Human CstF-50.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42895064105139,"sku":"BS2036-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42895064137907,"sku":"BS2036-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_f310b5d3-3d61-4db5-b652-90dccbefd067.jpg?v=1702573188","url":"https:\/\/afsbio.com\/products\/cstf-50-r3-polyclonal-antibody-bs2036","provider":"AFSBio Inc.","version":"1.0","type":"link"}