{"product_id":"cul1-y761-polyclonal-antibody-bs1081","title":"CUL1 (Y761) Polyclonal Antibody-BS1081","description":"\u003cp\u003eCUL1 (Y761) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1081-50, BS1081-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q13616\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eBackground: CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1\/CUL-1\/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Cullin 1 (Y761) polyclonal antibody detects endogenous levels of Cullin 1 protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of CUL1 (Y761) pAb at 1:500 dilution\u003cbr\u003e\nLane 1: The Brain tissue lysate of Rat (40ug)\u003cbr\u003e\nLane 2: The Lung tissue lysate of Rat (40ug)\u003cbr\u003e\nLane 3: The Lung tissue lysate of Mouse (40ug)\u003cbr\u003e\nLane 4: A549 whole cell lysate (40ug)\u003cbr\u003e\nLane 5: HCC827 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of CUL-1 (Y761) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 90 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CUL 1; CUL-1; CUL1; CUL1; Cullin-1; Cullin1; MGC149834; MGC149835;\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 728-780 of Human CUL-1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953206595763,"sku":"BS1081-50","price":254.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953206628531,"sku":"BS1081-100","price":442.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b19700cc-e010-4d64-a52c-510722acee53.jpg?v=1705056750","url":"https:\/\/afsbio.com\/products\/cul1-y761-polyclonal-antibody-bs1081","provider":"AFSBio Inc.","version":"1.0","type":"link"}