{"product_id":"cul5-polyclonal-antibody-bs7559","title":"CUL5 Polyclonal Antibody-BS7559","description":"\u003cp\u003eCUL5 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS7559-50, BS7559-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q93034\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:2000\u003cbr\u003e\nIF: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. Cul-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1\/Cul-1\/F-box protein complex) consists of Skp1 associating with both Cul-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. Cul-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: CUL5 polyclonal antibody detects endogenous levels of CUL5 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~91 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of CUL5 polyclonal antibody, Immunofluorescence analysis of U2OS cells, using CUL5 polyclonal antibody\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CUL 5; \u003cbr\u003e\nCUL-5; \u003cbr\u003e\nCUL5; \u003cbr\u003e\nCUL5_HUMAN; \u003cbr\u003e\nCullin-5 (vasopressin-activated calcium-mobilizing receptor-1); \u003cbr\u003e\nCullin-5; \u003cbr\u003e\nCullin5; \u003cbr\u003e\nVACM 1; \u003cbr\u003e\nVACM-1; \u003cbr\u003e\nVACM1; \u003cbr\u003e\nVasopressin activated calcium mobilizing receptor 1; \u003cbr\u003e\nVasopressin activated calcium mobilizing receptor; \u003cbr\u003e\nVasopressin-activated calcium-mobilizing receptor 1; \u003cbr\u003e\nVasopressin-activated calcium-mobilizing receptor-1;\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Recombinant full length Human CUL5.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989564428467,"sku":"BS7559-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989564461235,"sku":"BS7559-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_7887bd25-2625-4779-9de0-ffd7dac82610.jpg?v=1706095424","url":"https:\/\/afsbio.com\/products\/cul5-polyclonal-antibody-bs7559","provider":"AFSBio Inc.","version":"1.0","type":"link"}