{"product_id":"cyclin-d3-g277-polyclonal-antibody-ap0379","title":"Cyclin D3 (G277) Polyclonal Antibody-AP0379","description":"\u003cp\u003eCyclin D3 (G277) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: AP0379-50, AP0379-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P30281\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: The inactive ternary complex of cyclin D\/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase. The active complex of cyclin D\/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1\/S-phase gene expression. Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation.Although the D-type cyclins are not fully redundant, cyclin D3, like D1, plays a prominent role in differentiation and proliferation, which correlates with higher expression levels of cyclin D3 in various cancers.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Cyclin D3 (G277) polyclonal antibody detects endogenous levels of Cyclin D3 protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Cyclin D3 (G277) polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLane1:HEK293T whole cell lysate\u003cbr\u003e\nLane2:Raw264.7 whole cell lysate\u003cbr\u003e\nLane3:H9C2 whole cell lysate\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 34 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CCND 3; CCND3; CyclinD3; Cyclin-D3; D3 type cyclin; G1 S specific cyclin D3; G1\/S specific cyclin D3; G1\/S-specific cyclin-D3;\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 249-299 of Human Cyclin D3.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910472569011,"sku":"AP0379-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910472601779,"sku":"AP0379-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_38400097-0f90-436a-a8ea-c556e9305fbe.jpg?v=1703093093","url":"https:\/\/afsbio.com\/products\/cyclin-d3-g277-polyclonal-antibody-ap0379","provider":"AFSBio Inc.","version":"1.0","type":"link"}