{"product_id":"cyclin-e1-phospho-t395-polyclonal-antibody-bs4057","title":"Cyclin E1 (phospho-T395) Polyclonal Antibody-BS4057","description":"\u003cp\u003eCyclin E1 (phospho-T395) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS4057-50, BS4057-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P24864\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eCyclin E1 (phospho-T395) Polyclonal Antibody-BS4057\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1\/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: p-Cyclin E1 (T395) polyclonal antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at Thr395\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 48 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of p-Cyclin E1 (T395) pAb at 1:500 dilution\u003cbr\u003e\n Lane 1: A549 whole cell lysate (40ug) \u003cbr\u003e\n Lane 2: HepG2 whole cell lysate (40ug) \u003cbr\u003e\n Lane 3: C6 whole cell lysate (40ug) \u003cbr\u003e\n Lane 4: BV2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-Cyclin E1 (T395) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: G1\/S-specific cyclin-E1; CCNE1; CCNE\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human Cyclin E1 around the phosphorylation site of Threonine 395.\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957295485107,"sku":"BS4057-50","price":276.71,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957295517875,"sku":"BS4057-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_33dbf13c-37c3-4ad4-a0c6-4e345e0f7a69.jpg?v=1705144492","url":"https:\/\/afsbio.com\/products\/cyclin-e1-phospho-t395-polyclonal-antibody-bs4057","provider":"AFSBio Inc.","version":"1.0","type":"link"}