{"product_id":"cyclin-h-monoclonal-antibody-mb66662","title":"Cyclin H Monoclonal Antibody-MB66662","description":"\u003cp\u003eCyclin H Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66662-50, MB66662-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P51946\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cyclin H belongs to a conserved cyclin family that plays a critical role in the regulation of cell cycle dependent kinases (CDKs) necessary for cell cycle progression. In general, the activity of CDKs requires the binding of appropriate cyclins as well as phosphorylation driven by Cdk-activating kinase (CAK). Cyclin H is part of the CAK complex that includes the kinase CDK7, and an assembly factor p36\/Mat1, which enhances binding between cyclin H and CDK7 and increases activity. CAK regulates progression through the cell cycle by activating cdc2, CDK2, and CDK4 kinases through phosphorylation of a critical threonine residue in the T-loop of the CDK-cyclin complexes. The CAK complex can exist either in its free form or in association with transcription factor IIH (TFIIH) which can affect its substrate specificity. When bound to TFIIH, CAK preferentially phosphorylates the carboxy-terminal domain of RNA polymerase II, providing a link between cell cycle control, transcriptional regulation, and DNA repair.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of Cyclin H protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Cyclin H expression in Jurkat (A), K562 (B), Hela (C), NIH3T3 (D) whole cell lysates.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 36 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Cyclin-H; MO15-associated protein; p34; p37\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Purified recombinant human Cyclin H protein fragments expressed in E.coli.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42906366673075,"sku":"MB66662-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42906366705843,"sku":"MB66662-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_fd766def-74cd-413f-bf57-d6b097c2e487.jpg?v=1702918786","url":"https:\/\/afsbio.com\/products\/cyclin-h-monoclonal-antibody-mb66662","provider":"AFSBio Inc.","version":"1.0","type":"link"}