{"product_id":"cytochrome-p450-3a4-monoclonal-antibody-mb66848","title":"Cytochrome P450 3A4 Monoclonal Antibody-MB66848","description":"\u003cp\u003eCytochrome P450 3A4 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66848-50, MB66848-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgM. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P08684\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000)\u003c\/p\u003e\n\n\u003cp\u003eBackground: A cytochrome P450 monooxygenase involved in the metabolism of sterols, steroid hormones, retinoids and fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds .Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C-16 position.Plays a role in the metabolism of androgens, particularly in oxidative deactivation of testosterone.Metabolizes testosterone to less biologically active 2beta- and 6beta-hydroxytestosterones .Contributes to the formation of hydroxycholesterols (oxysterols), particularly A-ring hydroxylated cholesterol at the C-4beta position, and side chain hydroxylated cholesterol at the C-25 position, likely contributing to cholesterol degradation and bile acid biosynthesis Catalyzes bisallylic hydroxylation of polyunsaturated fatty acids (PUFA) .Catalyzes the epoxidation of double bonds of PUFA with a preference for the last double bond.Metabolizes endocannabinoid arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling .Plays a role in the metabolism of retinoids. Displays high catalytic activity for oxidation of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA).Further metabolizes atRA toward 4-hydroxyretinoate and may play a role in hepatic atRA clearance .Responsible for oxidative metabolism of xenobiotics. Acts as a 2-exo-monooxygenase for plant lipid 1,8-cineole.Metabolizes the majority of the administered drugs. Catalyzes sulfoxidation of the anthelmintics albendazole and fenbendazole .Hydroxylates antimalarial drug quinine.Acts as a 1,4-cineole 2-exo-monooxygenase .Also involved in vitamin D catabolism and calcium homeostasis. Catalyzes the inactivation of the active hormone calcitriol.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: This antibody is purified through a protein G column.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of Cytochrome P450 3A4 protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Cytochrome P450 3A4 expression in T47D (A) whole cell lysates.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 50 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CYP3A3; Cytochrome P450 3A4; 1,8-cineole 2-exo-monooxygenase; Albendazole monooxygenase; Albendazole sulfoxidase; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase; Quinine 3-monooxygenase; Taurochenodeoxycholate 6-alpha-hydroxylase\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 3A4. The exact sequence is proprietary.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42906385973427,"sku":"MB66848-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42906386006195,"sku":"MB66848-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_773bfb6f-fd76-44e8-951b-659cfa5c4f55.jpg?v=1702918952","url":"https:\/\/afsbio.com\/products\/cytochrome-p450-3a4-monoclonal-antibody-mb66848","provider":"AFSBio Inc.","version":"1.0","type":"link"}