{"product_id":"cytokeratin-12-krt12-polyclonal-antibody-bs75934","title":"Cytokeratin 12 (KRT12) Polyclonal Antibody-BS75934","description":"\u003cp\u003eCytokeratin 12 (KRT12) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers:  BS75934-50, BS75934-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q99456\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e \n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using Cytokeratin 12 ) antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% nonfat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 1s.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 56kDa\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: KRT12; K12\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human Cytokeratin 12 (Cytokeratin 12 (KRT12))(NP_000214.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42920338030771,"sku":"BS75934-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42920338063539,"sku":"BS75934-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_eb192e39-5c8b-4cd2-9825-2b3a107aa568.jpg?v=1703682849","url":"https:\/\/afsbio.com\/products\/cytokeratin-12-krt12-polyclonal-antibody-bs75934","provider":"AFSBio Inc.","version":"1.0","type":"link"}