{"product_id":"cytokeratin-14-polyclonal-antibody-bs90376","title":"Cytokeratin 14 Polyclonal Antibody-BS90376","description":"\u003cp\u003eCytokeratin 14 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90376-50, BS90376-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P02533(Human) Q61781(Mouse) Q6IFV1(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:1,000-1:2,000\u003cbr\u003e\nICC:1:100-1:500\u003cbr\u003e\nIHC:1:100-1:500\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed in pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. In Bowen’s disease, the characteristic malignancy of the epidermis exhibits distinct expression patterns of Cytokeratin 14. The gene encoding human Cytokeratin 14 maps to chromosome 17q12-21. Mutations in this gene lead to epidermolysis bullosa simplex, an inheritied skin disorder characterized by skin blistering due to basal keratinocyte fragility.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Cytokeratin 14 polyclonal antibody detects endogenous levels of Cytokeratin 14 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 53 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Cytokeratin 14 on different lysates using anti-Cytokeratin 14 antibody at 1\/1,000 dilution. \u003cbr\u003e\n Positive control: \u003cbr\u003e\n Lane 1: A431 \u003cbr\u003e\n Lane 2: Human skin, ICC staining Cytokeratin 14 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CK-14, K14, KRT14, CK 14, CK-14, ck14, Cytokeratin 14, Cytokeratin-14, Cytokeratin14, Dowling Meara, EBS3, EBS4, Epidermolysis bullosa simplex, K14, K1C14, Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner), Keratin 14, Keratin, Keratin type I cytoskeletal 14, Keratin, type I cytoskeletal 14, Keratin-14, Keratin14, Koebner, Krt 14, Krt14, NFJ, OTTHUMP00000164624, type I cytoskeletal 14,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979804479667,"sku":"BS90376-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979804512435,"sku":"BS90376-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_ec20a71c-66b4-4b80-8c87-c9458c5ae28d.jpg?v=1705752934","url":"https:\/\/afsbio.com\/products\/cytokeratin-14-polyclonal-antibody-bs90376","provider":"AFSBio Inc.","version":"1.0","type":"link"}