{"product_id":"ddddk-tag-1a8-monoclonal-antibody-hrp-ap0007mh","title":"DDDDK-tag (1A8) Monoclonal Antibody-HRP-AP0007MH","description":"\u003cp\u003eDDDDK-tag (1A8) Monoclonal Antibody-HRP\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: AP0007MH-50, AP0007MH-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB:1:1000~1:10000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase. The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: DDDDK-tag (1A8) mAb-HRP detects over-expressed or recombinant proteins containing the DDDDK epitope tag.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: N\/A\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of DDDDK-tag Mouse Antibody at 1:2000 dilution\u003cbr\u003e\n Lane 1: HEK293T whole cell lysate, transfected with HA-DDDDK-p62 48h (3uL)\u003cbr\u003e\n Lane 2: HEK293T whole cell lysate (40ug)\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag,ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDK\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Synthetic peptide DDDDK-tag.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: HRP\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42987091099827,"sku":"AP0007MH-50","price":263.54,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42987091132595,"sku":"AP0007MH-100","price":424.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_67776861-1889-4eaa-874f-134c64438a86.jpg?v=1706008956","url":"https:\/\/afsbio.com\/products\/ddddk-tag-1a8-monoclonal-antibody-hrp-ap0007mh","provider":"AFSBio Inc.","version":"1.0","type":"link"}