{"product_id":"ddddk-tag-binds-to-flag-sequnence-rabbit-pab-es1078","title":"DDDDK-Tag (binds to flag sequnence) rabbit pAb - ES1078","description":"\u003cp\u003eDDDDK-Tag (binds to flag sequnence) rabbit pAb\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES1078-50, ES1078-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Name: DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB; ELISA; IP; IF\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/1000 - 1\/3000. ELISA: 1:5000-20000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Species independent\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: DDDDK synthetic peptide conjugated to KLH.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"50μL","offer_id":45201787977907,"sku":"ES1078-50","price":207.2,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45201788010675,"sku":"ES1078-100","price":347.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_68b1326f-4299-4e53-9372-f3392277b1be.png?v=1767111821","url":"https:\/\/afsbio.com\/products\/ddddk-tag-binds-to-flag-sequnence-rabbit-pab-es1078","provider":"AFSBio Inc.","version":"1.0","type":"link"}