{"product_id":"ddit3-polyclonal-antibody-bs90400","title":"DDIT3 Polyclonal Antibody-BS90400","description":"\u003cp\u003eDDIT3 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90400-50, BS90400-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P35638(Human) P35639(Mouse)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500-1:2,000\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: DDIT3 also named GADD 153 which has been described as a growth arrest and DNA damage-inducible gene that encodes a C\/EBP-related nuclear protein. This protein has also been designated C\/EBP-homologous protein (CHOP-10). GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations. GADD 153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C\/EBP proteins to direct GADD 153 dimers away from \"classical\" C\/EBP binding sites, recognizing instead unique \"nonclassical\" sites. Thus GADD 153 acts as a negative modulator of C\/EBP-like proteins in certain terminally differentiated cells, similar to the regulatory function of Id on the activity of Myo D and Myo D-related proteins involved in the development of muscle cells.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Peptide affinity purified.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: DDIT3 polyclonal antibody detects endogenous levels of DDIT3 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 27 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of DDIT3 on different lysates using anti-DDIT3 antibody at 1\/500 dilution. \u003cbr\u003e\n Positive control: \u003cbr\u003e\n Lane 1: Mouse testis tissue \u003cbr\u003e\n Lane 2: LOVO \u003cbr\u003e\n Lane 3: Mouse brain tissue, Flow cytometric analysis of HepG2 cells with DDIT3 antibody at 1\/50 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: DDIT-3, CHOP, CHOP-10, DDIT3, CHOP, CHOP10, GADD153, C\/EBP homologous protein, C\/EBP Homology Protein, C\/EBP zeta, C\/EBP-homologous protein 10, C\/EBP-homologous protein, CCAAT\/enhancer binding protein homologous protein, CEBPZ, CHOP 10, CHOP, CHOP-10, CHOP10, DDIT 3, DDIT-3, Ddit3, DDIT3, DNA Damage Inducible Transcript 3, DNA damage-inducible transcript 3 protein, GADD 153, GADD153, Growth Arrest and DNA Damage Inducible Protein 153, Growth arrest and DNA damage inducible protein GADD153, Growth arrest and DNA damage-inducible protein GADD153, MGC4154,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Synthetic peptide within human DDIT3 aa 70-110.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979805954227,"sku":"BS90400-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979805986995,"sku":"BS90400-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_240d155b-b91d-4d3b-a417-7134bb627907.jpg?v=1705752986","url":"https:\/\/afsbio.com\/products\/ddit3-polyclonal-antibody-bs90400","provider":"AFSBio Inc.","version":"1.0","type":"link"}