CALIXAR’s DDTAC reagent is a non-ionic detergent that can be used to solubilize, extract and stabilize membrane proteins (GPCRs, Ion channels, Transporters, ….).
Compound name: (DDLAC) Dodecylmercapto-S(poly(tris(hydroxymethyl) acrylamidomethane)
Catalogue number: DDTAC_250MG
Molec. Formula: na
MW: ≈1253 g/mol
Percent Composition: na
Physical state: White powder
Purity (HPLC, 214nm): nd
Retention time (RP18 HPLC)b: tR = 11.8 min
CMC: 0.25 mM
Exact mass: ~0.15 mM
Stability: Store in <-20°C freezer for up to one year
Solubility Structure: Soluble in water (15mM), methanol and DMSO
CALIXAR used a unique & tailor-made procedure
CALIXAR’s DDTAC is a very mild detergent used to extract native membrane proteins. DDTAC can solubilize and stabilize more efficiently fragile membrane proteins of high medical relevance.
Our DDTAC is non-ionic and therefore not sensitive to ionic strengths or pH variations and can be customized to solubilize and stabilize specific membrane proteins.
DDTAC helps clients to produce lipid detergents, mixed micelles and or protein detergent micelles. CALIXAR allows biochemists, structural biologists, pharmacologists and or virologists to work with high-quality DDTAC to extract, solubilize and stabilize native and functional membrane proteins in the solution for research purposes, drug discovery and or target validation.
CALIXAR’s DDTAC is non-ionic and therefore is not sensitive to ionic strength or pH variations and is a very powerful way to extract, solubilize and stabilize particular membrane proteins.
CALIXAR’s DDTAC extracts high-quality membrane proteins that are used in research, drug discovery projects and structural studies. DDTAC is used by pharmaceutical and biotechnology companies, as well as life science academic teams (biochemists, structural biologists, pharmacologists, virologists).
- Antibodies (including nanobodies, scaffold proteins, aptamers)
- Small molecules
- 3D Structures (cryoEM, XRay crystallography, NMR, SANS, SAXS)
- Drug discovery (Screening: HTS, FBDD, SBDD; Hit and lead validation)
- Antibody discovery (Immunization and display technologies)
- Clinical stage (drug validation on reliable native DDLAC)
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
Hydrogenated and fluorinated surfactants derived from Tris(hydroxymethyl)-acrylamidomethane allow the purification of a highly active yeast F1-F0 ATP-synthase with an enhanced stability.
Talbot J.-C., et al. 2009