{"product_id":"ddx21-monoclonal-antibody-mb10822","title":"DDX21 Monoclonal Antibody-MB10822","description":"\u003cp\u003eDDX21 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB10822-50, MB10822-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9NR30\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1\/500-1\/1000  IF: 1\/50-1\/200\u003c\/p\u003e  \n\n\u003cp\u003eBackground: RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II) (PubMed:25470060). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs (PubMed:25470060). In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes (PubMed:25470060, PubMed:25477391). In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes (PubMed:25470060). Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77' (PubMed:11823437, PubMed:25260534). Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase) (PubMed:9461305). Involved in rRNA processing (PubMed:14559904, PubMed:18180292). May bind to specific miRNA hairpins (PubMed:28431233).\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Affinity Purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IgG\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Calculated MW: 87 kDa; Observed MW: 87 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Immunocytochemistry analysis of DDX21 in 293T using DDX21 antibody, and DAPI., Western blot analysis of DDX21 in Hela lysates using DDX21 antibody.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: GUA; GURDB; RH-II\/GU; RH-II\/GuA\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide of human DDX21\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42970493321395,"sku":"MB10822-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42970493354163,"sku":"MB10822-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_aab49364-ad49-4548-a347-2239063d2ab8.jpg?v=1705526346","url":"https:\/\/afsbio.com\/products\/ddx21-monoclonal-antibody-mb10822","provider":"AFSBio Inc.","version":"1.0","type":"link"}