{"product_id":"ddx3x-monoclonal-antibody-mb66592","title":"DDX3X Monoclonal Antibody-MB66592","description":"\u003cp\u003eDDX3X Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66592-50, MB66592-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O00571\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat, Monkey\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC, IP, , ChIP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IF\/ICC (1\/50 - 1\/100), IP (1\/10 - 1\/50), ChIP (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003eBackground: The DEAD box family of RNA helicases is characterized in part by a common D-E-A-D amino acid motif. The family is composed of a growing number of proteins found in a wide range of organisms from bacteria to mammals. DEAD helicases have distinct biological functions in RNA metabolism and ribonucleoprotein (RNP) processing (reviewed in 1,2).\u003cbr\u003e\nDDX3 is a DEAD box family RNA helicase with diverse cellular functions. DDX3 is required for nuclear export of HIV-1 viral transcripts, possibly in a complex with the viral Rev protein and host cofactor CRM1. DDX3 is required for hepatitis C virus (HCV) RNA replication and its expression is downregulated in hepatitis B virus (HBV) associated hepatocellular carcinoma (HCC).\u003cbr\u003e\nRecent evidence suggests that DDX3 functions as a tumor suppressor protein. Its expression inhibits tumor cell colony formation and increases expression of the cdk inhibitor p21 Waf1\/Cip1. Low DDX3 expression has been shown in HCC, and aberrant subcellular localization occurs in many squamous cell carcinomas. Reduced DDX3 expression in cultured cells causes a diminished dependence on serum for cell proliferation and changes in cyclin D1 and p21 Waf1\/Cip1 expression. DDX3 is phosphorylated at Thr204 and Thr323 by the mitotic cyclin dependent kinase, cyclin B\/cdc2. This phosphorylation is thought to cause a loss of DDX3 function and a concomitant repression of ribosome biogenesis and translation in mitosis.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of DDX3X protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 75 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of DDX3X expression in Hela (A), NIH3T3 (B), C6 (C), COS7 (D), K562 (E), Jurkat (F) whole cell lysates., Immunofluorescent analysis of DDX3X staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: DBX; DDX3; ATP-dependent RNA helicase DDX3X; DEAD box protein 3 X-chromosomal; DEAD box X isoform; Helicase-like protein 2; HLP2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Purified recombinant human DDX3 protein fragments expressed in E.coli.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989544603827,"sku":"MB66592-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989544636595,"sku":"MB66592-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_972df05f-a20c-414a-a5d1-235230b23dd2.jpg?v=1706095097","url":"https:\/\/afsbio.com\/products\/ddx3x-monoclonal-antibody-mb66592","provider":"AFSBio Inc.","version":"1.0","type":"link"}