{"product_id":"dgk-zeta-rabbit-monoclonal-antibody-mb66433","title":"DGK zeta Rabbit Monoclonal Antibody-MB66433","description":"\u003cp\u003eDGK zeta Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66433-50, MB66433-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q13574\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IP (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Diacylglycerol kinase that converts diacylglycerol\/DAG into phosphatidic acid\/phosphatidate\/PA and regulates the respective levels of these two bioactive lipids.\u003cbr\u003e\nThereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes.\u003cbr\u003e\nAlso plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species.\u003cbr\u003e\nCan also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group.\u003cbr\u003e\nThe biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol (By similarity).\u003cbr\u003e\nBy generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization.\u003cbr\u003e\nThrough the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane (By similarity).\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of DGK zeta protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of DGK zeta expression in Hela (A), A549 (B), HL60 (C), U2OS (D), U87MG (E), mouse brain (F), rat brain (G) whole cell lysates.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 120 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: DAGK6; Diacylglycerol kinase zeta; DAG kinase zeta; Diglyceride kinase zeta; DGK-zeta\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein of human DGKZ\/DGK-zeta\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42906361135283,"sku":"MB66433-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42906361168051,"sku":"MB66433-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_d3aca90e-24a0-4f82-bf23-867b74f7fb0c.jpg?v=1702918671","url":"https:\/\/afsbio.com\/products\/dgk-zeta-rabbit-monoclonal-antibody-mb66433","provider":"AFSBio Inc.","version":"1.0","type":"link"}