{"product_id":"dhvd3-1-25-dihydroxyvitamin-d3-elisa-kit-elk8091","title":"DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit - ELK8091","description":"\u003cp\u003eDHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK8091-48, ELK8091-96, ELK8091-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25- (OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 48.8 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 156.25-10000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Metabolic pathway; Bone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212354126003,"sku":"ELK8091-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212354158771,"sku":"ELK8091-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200790618291,"sku":"ELK8091-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_eaae6bd4-71dd-4ed5-959d-af4446724f5b.png?v=1767063875","url":"https:\/\/afsbio.com\/products\/dhvd3-1-25-dihydroxyvitamin-d3-elisa-kit-elk8091","provider":"AFSBio Inc.","version":"1.0","type":"link"}