{"product_id":"dna-pol-ι-polyclonal-antibody-ra24258","title":"DNA pol ι Polyclonal Antibody - RA24258","description":"\u003cp\u003eDNA pol ι Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RA24258-50, RA24258-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: catalytic activity:Deoxynucleoside triphosphate + DNA (n) = diphosphate + DNA (n+1) .,cofactor:Magnesium.,domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.,function:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.,similarity:Belongs to the DNA polymerase type-Y family.,similarity:Contains 1 umuC domain.,subcellular location:Accumulates at replication forks after DNA damage.,subunit:Binds REV1L (By similarity) . Binds POLH.,tissue specificity:Ubiquitous. Highly expressed in testis.,\u003c\/p\u003e\n\n\u003cp\u003eCondition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eStorage Instructions: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eRecommended dilutions: Western Blot: 1\/500 - 1\/2000. ELISA: 1\/40000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: POLI; RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB; ELISA\u003c\/p\u003e\n\n\u003cp\u003eSpecies Cross-Reactivity: Human; Rat; Mouse\u003c\/p\u003e\n\n\u003cp\u003eGeneID (Human): 11201\u003c\/p\u003e\n\n\u003cp\u003eProtein MW (KDa): 85\u003c\/p\u003e\n\n\u003cp\u003eSWISS: Q9UNA4\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Reed Bio","offers":[{"title":"50μL","offer_id":45121244004531,"sku":"RA24258-50","price":336.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45121244037299,"sku":"RA24258-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/logo_8664a0ff-d81d-447e-8c03-f3c1389ab0b6.png?v=1773345643","url":"https:\/\/afsbio.com\/products\/dna-pol-%ce%b9-polyclonal-antibody-ra24258","provider":"AFSBio Inc.","version":"1.0","type":"link"}