{"product_id":"dog-et-1-endothelin1-elisa-kit-elk5700","title":"Dog ET-1 (Endothelin1) ELISA Kit - ELK5700","description":"\u003cp\u003eDog ET-1 (Endothelin1) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK5700-48, ELK5700-96, ELK5700-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 2.65 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 500 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 7.82-500 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Endocrinology; Cardiovascular biology;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: P13206\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213601996979,"sku":"ELK5700-48","price":576.8,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213602029747,"sku":"ELK5700-96","price":823.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45201346396339,"sku":"ELK5700-5x96","price":3498.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_6d990556-20d8-4521-9b2d-ee2d46afd065.png?v=1767093518","url":"https:\/\/afsbio.com\/products\/dog-et-1-endothelin1-elisa-kit-elk5700","provider":"AFSBio Inc.","version":"1.0","type":"link"}