{"product_id":"dog-nt-probnp-n-terminal-pro-brain-natriuretic-peptide-elisa-kit-elk0199","title":"Dog NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit - ELK0199","description":"\u003cp\u003eDog NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0199-48, ELK0199-96, ELK0199-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: NT-Pro-BNP; N-BNP; ANFB_HUMAN; BNP (1-32); BNP (5-29); BNP-32; Gamma-brain natriuretic peptide; nppb\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 24.8 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 5000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 78.13-5000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Endocrinology; Cardiovascular biology;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211445862579,"sku":"ELK0199-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211445895347,"sku":"ELK0199-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200631267507,"sku":"ELK0199-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_fccbc990-0a28-4d5c-adcc-2e88be2bc7b3.png?v=1767060297","url":"https:\/\/afsbio.com\/products\/dog-nt-probnp-n-terminal-pro-brain-natriuretic-peptide-elisa-kit-elk0199","provider":"AFSBio Inc.","version":"1.0","type":"link"}