{"product_id":"dut-polyclonal-antibody-e-ab-67503","title":"DUT Polyclonal Antibody - E-AB-67503","description":"\u003cp\u003eDUT Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 60μL, 120μL, 200μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-AB-67503-60, E-AB-67503-120, E-AB-67503-200\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: DUT\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: DUT; dUTPase\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Epigenetics and Nuclear Signaling\u003c\/p\u003e\n\n\u003cp\u003eConjugation: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eSpecies Reactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplication: WB, IHC, IF\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n \n\n\u003cp\u003eUNIProt ID: P33316\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1mg\/mL\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human DUT (NP_001020419.1).\u003c\/p\u003e\n\n\u003cp\u003eBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.\u003c\/p\u003e\n\n\u003cp\u003ePurification Method: Affinity purification\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB \t1:500-1:1000   IHC \t1:50-1:200   IF \t1:100-1:200\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 17kDa\/26kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 24kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: -20°C\/One year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: Ice bag\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"60μL","offer_id":43146445258931,"sku":"E-AB-67503-60","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"120μL","offer_id":43146445291699,"sku":"E-AB-67503-120","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"200μL","offer_id":43146445324467,"sku":"E-AB-67503-200","price":742.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_9cf41b80-8867-4d95-9065-3a13bc73d4a8.png?v=1710295692","url":"https:\/\/afsbio.com\/products\/dut-polyclonal-antibody-e-ab-67503","provider":"AFSBio Inc.","version":"1.0","type":"link"}