{"product_id":"ebp50-polyclonal-antibody-bs90442","title":"EBP50 Polyclonal Antibody-BS90442","description":"\u003cp\u003eEBP50 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90442-50, BS90442-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O14745(Human) Q9JJ19(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:1,000-1:2,000\u003cbr\u003e\nIHC:1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The Na+\/H+ exchange protein (NHE3) functions in transepithelial Na+ absorption and is primarily expressed in the intestinal and renal brush border membrane. NHE3 regulatory factor 1 (NHERF-1) interacts with NHE3 through two PDZ (for PSD-95, Discs-large and ZO-1 homology) domains, which are protein-protein interaction modules that associate with specific carboxy-terminal motifs on target proteins. Also known as EBP50, NHERF-1 facilitates cAMP inhibition of NHE3 to decrease Na+ adsorption. NHERF-1 functions as a scaffold for an essential multiprotein complex of Ezrin and NHE3 for cAMP-mediated phosphorylation and consequent inhibition of NHE3. The amino-terminal PDZ domain regulates the dimerization of NHERF-1 in vivo. G protein-coupled receptor kinase 6A phosphorylates NHERF-1 at Ser 289 via a PDZ domain-mediated interaction. NHERF-2, also known as E3KARP, is a ubiquitously expressed protein which also functions in NHE2 regulation.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: EBP50 polyclonal antibody detects endogenous levels of EBP50 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 50 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of EBP50 on MCF-7 cells lysates using anti-EBP50 antibody at 1\/1,000 dilution., Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: NHERF-1, EBP50, SLC9A3R1, EBP 50, EBP50, Ezrin radixin moesin binding phosphoprotein 50, Ezrin-radixin-moesin-binding phosphoprotein 50, Na(+)\/H(+) exchange regulatory cofactor NHE RF, Na(+)\/H(+) exchange regulatory cofactor NHE-RF1, Na+\/H+ exchange regulatory co factor, NHERF 1, NHERF, NHERF-1, NHERF1, NHRF1, NPHLOP2, Regulatory cofactor of Na(+)\/H(+) exchanger, Slc9a3r1, Sodium hydrogen exchanger regulatory factor 1, Sodium-hydrogen exchanger regulatory factor 1, Sodium\/hydrogen exchanger regulatory factor 1, Solute carrier family 9 (sodium\/hydrogen exchanger) member 3 regulator 1, Solute carrier family 9 (sodium\/hydrogen exchanger), isoform 3 regulatory factor 1, Solute carrier family 9 isoform 3 regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1, Solute carrier family 9 member 3 regulator 1,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979808510131,"sku":"BS90442-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979808542899,"sku":"BS90442-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_98b1cc9d-9c2d-4bed-8feb-afe3f7942e4e.jpg?v=1705753075","url":"https:\/\/afsbio.com\/products\/ebp50-polyclonal-antibody-bs90442","provider":"AFSBio Inc.","version":"1.0","type":"link"}