{"product_id":"ect2-monoclonal-antibody-mb67210","title":"ECT2 Monoclonal Antibody-MB67210","description":"\u003cp\u003eECT2 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB67210-50, MB67210-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9H8V3\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Epithelial cell transforming sequence 2 (ECT2), a guanine nucleotide exchange factor (GEF), catalyzes the exchange of GDP for GTP. The nuclear GEF activity of ECT2 is essential for Kras-Trp53 lung tumorigenesis. This transformation requires ECT2-mediated rRNA synthesis. In addition, PKCι-dependent phosphorylation of ECT2 is necessary for the oncogenic activity of this protein. Furthermore, ECT2 has been implicated as a cancer driver gene in certain subtypes of liver cancers. Research studies also show that, during matrix-directed cell migration, low membrane tension stimulates the formation of caveolae at the rear end of moving cells. ECT2 is recruited to the caveolae and activates RhoA, causing F-actin organization and rear retraction.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: This antibody is purified through a protein G column.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of ECT2 protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of ECT2 expression in 293T (A) whole cell lysates.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 104 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Protein ECT2; Epithelial cell-transforming sequence 2 oncogene\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ECT2. The exact sequence is proprietary.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42906419003571,"sku":"MB67210-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42906419036339,"sku":"MB67210-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_64a69f6b-d528-4db5-84be-db26cf2472de.jpg?v=1702919248","url":"https:\/\/afsbio.com\/products\/ect2-monoclonal-antibody-mb67210","provider":"AFSBio Inc.","version":"1.0","type":"link"}