{"product_id":"edg1-polyclonal-antibody-bs90444","title":"EDG1 Polyclonal Antibody-BS90444","description":"\u003cp\u003eEDG1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90444-50, BS90444-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P21453(Human) O08530(Mouse) P48303(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF, IHC, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:1,000-1:5,000\u003cbr\u003e\nICC:1:100-1:500\u003cbr\u003e\nIHC:1:50-1:200\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: The EDG (endothelial differentiation gene) family of G protein coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high affinity. EDG-6 is a low affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. EDG-6, which is expressed in lymphoid and hematopoietic tissues and in lung, signals through G(i\/o) proteins, which activate growth related pathways\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: EDG1 polyclonal antibody detects endogenous levels of EDG1 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 43 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of EDG1 on SH-SY5Y cells lysates using anti-EDG1 antibody at 1\/1,000 dilution., ICC staining EDG1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: S1P receptor 1, S1P1, S1P receptor Edg-1, S1PR1, CD363, CHEDG 1, CHEDG1, D1S3362, ECGF 1, ECGF1, EDG 1, EDG1, endothelial differentiation G protein coupled receptor 1, Endothelial differentiation G-protein coupled receptor 1, Endothelial differentiation sphingolipid G protein coupled receptor 1, FLJ58121, G protein coupled sphingolipid receptor, g protein-coupled receptor edg-1, S1P receptor 1, S1P receptor Edg 1, S1P receptor Edg-1, S1P receptor Edg1, S1P(1) receptor, S1P1, s1pr1, S1PR1, sphingolipid g-protein-coupled receptor 1, Sphingosine 1 phosphate receptor Edg 1, Sphingosine 1 phosphate receptor EDG1, sphingosine 1- phosphate receptor 1, Sphingosine 1-phosphate receptor 1, Sphingosine 1-phosphate receptor Edg-1, Sphingosine 1phosphate receptor type 1 S1P1,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42979808575667,"sku":"BS90444-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42979808608435,"sku":"BS90444-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_3290596f-3034-41a6-841b-bec126870c59.jpg?v=1705753077","url":"https:\/\/afsbio.com\/products\/edg1-polyclonal-antibody-bs90444","provider":"AFSBio Inc.","version":"1.0","type":"link"}