{"product_id":"eef2-monoclonal-antibody-mb66520","title":"EEF2 Monoclonal Antibody - MB66520","description":"\u003cp\u003eEEF2 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66520-50, MB66520-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. It has been shown that phosphorylation of eEF2 at threonine 56 by eEF2 kinase inhibits its activity. eEF2 kinase is normally dependent on Ca2+ ions and calmodμlin. eEF2 kinase can also be activated by PKA in response to elevated cAMP levels, which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellμlar Ca2+ or cAMP levels have been shown to resμlt in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. The inactive phosphorylated eEF2 can be converted to its active nonphosphorylated form by a protein phosphatase, most likely a form of protein phosphatase-2A (PP-2A). Insμlin, which activates protein synthesis in a wide range of cell types, induces rapid dephosphorylation of eEF2 through mTOR signaling and may involve modμlation of the activity of the PP-2A or the eEF2 kinase or both.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC, ChIP\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: EF2, Elongation factor 2, EF-2\u003c\/p\u003e\n\n\u003cp\u003eClonality: Monoclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Purified recombinant human eEF2 protein fragments expressed in E.coli.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB (1\/500 - 1\/1000), IF\/ICC (1\/50 - 1\/100), ChIP (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of EEF2 protein.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 95 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: AMPK Signaling Pathway\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: P13639\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45946407223475,"sku":"MB66520-50","price":274.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946407256243,"sku":"MB66520-100","price":457.5,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/eef2-monoclonal-antibody-mb66520","provider":"AFSBio Inc.","version":"1.0","type":"link"}