{"product_id":"eef2-phospho-t56-polyclonal-antibody-bs4059","title":"EEF2 (phospho-T56) Polyclonal Antibody-BS4059","description":"\u003cp\u003eEEF2 (phospho-T56) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS4059-50, BS4059-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P13639\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eEEF2 (phospho-T56) Polyclonal Antibody-BS4059\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved. EF-2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: p-EEF2 (T56) polyclonal antibody detects endogenous levels of EF-2 protein when phosphorylated at Thr56.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 95 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of p-EEF2 (T56) pAb at 1:500 dilution\u003cbr\u003e\n Lane 1: K562 whole cell lysate (40ug) \u003cbr\u003e\n Lane 2: HEK293T whole cell lysate (10ug) \u003cbr\u003e\n Lane 3: The Liver tissue lysate of Rat (40ug) \u003cbr\u003e\n Lane 4: The Liver tissue lysate of Mouse (40ug), Immunohistochemistry (IHC) analyzes of p-EEF2 (T56) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Elongation factor 2; EF-2; EEF2; EF2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 20-70 of Human eEF2.\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957295550643,"sku":"BS4059-50","price":276.71,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957295583411,"sku":"BS4059-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_355c2bee-c702-4e2f-8a55-c9ff8d60de52.jpg?v=1705144495","url":"https:\/\/afsbio.com\/products\/eef2-phospho-t56-polyclonal-antibody-bs4059","provider":"AFSBio Inc.","version":"1.0","type":"link"}