{"product_id":"egr1-polyclonal-antibody-bs80415","title":"EGR1 Polyclonal Antibody-BS80415","description":"\u003cp\u003eEGR1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS80415-50, BS80415-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P18146\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eEGR1 Polyclonal Antibody-BS80415\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:200 - 1:1000  |  IF\/ICC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 75KDa\/100KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using EGR1 antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Enhanced Kit.\u003cbr\u003e\nExposure time: 10s., Immunofluorescence analysis of U2OS cells using EGR1 Rabbit pAb at dilution of 1:250. Blue: DAPI for nuclear staining.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: EGR1; AT225; G0S30; KROX-24; NGFI-A; TIS8; ZIF-268; ZNF225\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human EGR1(NP_001955.1).\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42960143909043,"sku":"BS80415-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42960143941811,"sku":"BS80415-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_bf9d2364-4817-4453-8c5b-f0574910b6b8.jpg?v=1705231992","url":"https:\/\/afsbio.com\/products\/egr1-polyclonal-antibody-bs80415","provider":"AFSBio Inc.","version":"1.0","type":"link"}