{"product_id":"eif2b3-monoclonal-antibody-mb67223","title":"EIF2B3 Monoclonal Antibody-MB67223","description":"\u003cp\u003eEIF2B3 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB67223-50, MB67223-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9NR50\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IF\/ICC (1\/10 - 1\/50), FC (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: This antibody is purified through a protein G column.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of EIF2B3 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 56 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of EIF2B3 expression in HL60 (A), MCF7 (B), U2OS (C), K562 (D) whole cell lysates., Immunofluorescent analysis of EIF2B3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Translation initiation factor eIF-2B subunit gamma; eIF-2B GDP-GTP exchange factor subunit gamma\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EIF2B3. The exact sequence is proprietary.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989551812787,"sku":"MB67223-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989551845555,"sku":"MB67223-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_038fd14d-41c0-48d0-bdde-b994fc3abad1.jpg?v=1706095183","url":"https:\/\/afsbio.com\/products\/eif2b3-monoclonal-antibody-mb67223","provider":"AFSBio Inc.","version":"1.0","type":"link"}