{"product_id":"eif3a-polyclonal-antibody-e-ab-62282","title":"EIF3A Polyclonal Antibody - E-AB-62282","description":"\u003cp\u003eEIF3A Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 60μL, 120μL, 200μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-AB-62282-60, E-AB-62282-120, E-AB-62282-200\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: EIF3A\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: EIF3A; EIF3; EIF3S10; P167; TIF32; eIF3-p170; eIF3-theta; p180; p185\u003c\/p\u003e\n\n\u003cp\u003eConjugation: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eSpecies Reactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplication: WB, IF\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q14152\u003c\/p\u003e\n\n\u003cp\u003eBackground: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815).\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/mL\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human EIF3A\u003c\/p\u003e\n\n\u003cp\u003eBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.\u003c\/p\u003e\n\n\u003cp\u003ePurification Method: Affinity purification\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB 1:500-1:1000, IF 1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 162kDa\/166kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 180kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: -20°C\/One year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: Ice bag\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"60μL","offer_id":43148641173683,"sku":"E-AB-62282-60","price":280.0,"currency_code":"CAD","in_stock":true},{"title":"120μL","offer_id":43148641206451,"sku":"E-AB-62282-120","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"200μL","offer_id":43148641239219,"sku":"E-AB-62282-200","price":742.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_0e4d3f5a-e18a-4907-8467-13770e4b709f.png?v=1710327180","url":"https:\/\/afsbio.com\/products\/eif3a-polyclonal-antibody-e-ab-62282","provider":"AFSBio Inc.","version":"1.0","type":"link"}