{"product_id":"eif4b-phospho-s422-polyclonal-antibody-bs4311","title":"eIF4B (phospho-S422) Polyclonal Antibody -BS4311","description":"\u003cp\u003eeIF4B (phospho-S422) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS4311-50, BS4311-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P23588\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eeIF4B (phospho-S422) Polyclonal Antibody -BS4311\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.It is phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1.phosphorylation enhances the affinity of EIF4B for the EIF3 complex\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: p-eIF4B (S422) polyclonal antibody detects endogenous levels of eIF4B protein only when phosphorylated at Ser422\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 80 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of p-eIF4B (S422) pAb at 1:500 dilution\u003cbr\u003e\n Lane 1: The Brain tissue lysate of Rat (40ug) \u003cbr\u003e\n Lane 2: K562 whole cell lysate (40ug) \u003cbr\u003e\n Lane 3: A375 whole cell lysate (40ug) \u003cbr\u003e\n Lane 4: HCT116 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-eIF4B (S422) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Eukaryotic translation initiation factor 4B; eIF-4B; EIF4B\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human eIF4B around the phosphorylation site of Serine 422.\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957298139315,"sku":"BS4311-50","price":276.71,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957298172083,"sku":"BS4311-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_ff2874ab-6313-4fd9-a5b4-1d6fefd3d757.jpg?v=1705144528","url":"https:\/\/afsbio.com\/products\/eif4b-phospho-s422-polyclonal-antibody-bs4311","provider":"AFSBio Inc.","version":"1.0","type":"link"}