{"product_id":"eif4e-monoclonal-antibody-mb10870","title":"eIF4E Monoclonal Antibody-MB10870","description":"\u003cp\u003eeIF4E Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB10870-50, MB10870-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P06730\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC-P, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1\/500-1\/1000  IHC: 1\/50-1\/100  IP: 1\/20\u003c\/p\u003e  \n\n\u003cp\u003eBackground: eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and\/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Affinity Purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IgG\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Calculated MW: 25 kDa; Observed MW: 25 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Immunohistochemistry analysis of paraffin-embedded mouse colon using eIF4E antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval., Western blot analysis of eIF4E in K562, rat Brain, C6, 3T3, Hela lysates using eIF4E antibody.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide of human eIF4E\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42970495189171,"sku":"MB10870-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42970495221939,"sku":"MB10870-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_9889924f-dfbd-4df1-be5a-1a7037d65be0.jpg?v=1705526388","url":"https:\/\/afsbio.com\/products\/eif4e-monoclonal-antibody-mb10870","provider":"AFSBio Inc.","version":"1.0","type":"link"}