{"product_id":"elisa-kit-for-anti-anti-mullerian-hormone-antibody-anti-amh-uaea228mu","title":"ELISA Kit for Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) - UAEA228Mu","description":"\u003cp\u003eELISA Kit for Anti-Anti-Mullerian Hormone Antibody (Anti-AMH)\u003c\/p\u003e\n\n\u003cp\u003eSize: 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: UAEA228Mu-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eTarget: Anti-Mullerian Hormone (Anti-AMH)\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eDetection range: 3.12-200ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 1.19ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eUniprot: P27106\u003c\/p\u003e\n\n\u003cp\u003eGene ID: 11705\u003c\/p\u003e\n\n\u003cp\u003eFeatured Series Function: Detects protein (antigen)\u003c\/p\u003e\n\n\u003cp\u003eRecommended\/Predicted Sample Types: Serum, Plasma and other Biological Fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 2h, 30min\u003c\/p\u003e\n\n\u003cp\u003eMethod: Colormetric\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Reactive with Mouse AMH \/ Mullerian Hormone\u003c\/p\u003e\n\n\u003cp\u003eDetection principle: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSample Size: 50uL\u003c\/p\u003e\n\n\u003cp\u003eAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\n\n\u003cp\u003eStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\n\n\u003cp\u003eShelf-life: 12 months\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This assay has high sensitivity and excellent specificity for detection of Anti-Anti-Mullerian Hormone Antibody (Anti-AMH).\u003cbr\u003e\nNo significant cross-reactivity or interference between Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) and analogues was observed.\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) were tested 20 times on one plate, respectively.\u003cbr\u003e\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) were tested on 3 different plates, 8 replicates in each plate.\u003cbr\u003e\nCV(%) = SD\/meanX100\u003c\/p\u003e\n\n\u003cp\u003eStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\u003cbr\u003e\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\n\n\u003cp\u003eAssay procedure summary: 1. Prepare all reagents, samples and standards;\u003cbr\u003e\n2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;\u003cbr\u003e\n3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;\u003cbr\u003e\n4. Aspirate and wash 5 times;\u003cbr\u003e\n5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;\u003cbr\u003e\n6. Add 50µL Stop Solution. Read at 450nm immediately.\u003c\/p\u003e\n\n\u003cp\u003eTest principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) and unlabeled Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) (Standards or samples) with the pre-coated antibody specific to Anti-Anti-Mullerian Hormone Antibody (Anti-AMH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) in the sample.\u003c\/p\u003e\n\n\u003cp\u003eResearch Area: Endocrinology; Reproductive science; Hormone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"USCN","offers":[{"title":"Default Title","offer_id":45390119370931,"sku":"UAEA228Mu-96","price":630.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/USCN.png?v=1774797733","url":"https:\/\/afsbio.com\/products\/elisa-kit-for-anti-anti-mullerian-hormone-antibody-anti-amh-uaea228mu","provider":"AFSBio Inc.","version":"1.0","type":"link"}