{"product_id":"elisa-kit-for-c-ros-oncogene-1-receptor-tyrosine-kinase-ros1-usee273hu","title":"ELISA Kit for C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) - USEE273Hu","description":"ELISA Kit for C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1)\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eSize: 96T\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eCatalogue Number: USEE273Hu-96\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eCitations, Manuals and MSDS Available upon request.\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eTarget: C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1)\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eAlternative Names: MCF3; ROS; c-Ros-1; v-Ros Avian UR2 Sarcoma Virus Oncogene Homolog 1; Proto-oncogene tyrosine-protein kinase ROS; Receptor tyrosine kinase c-ros oncogene 1\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eTarget Species: Human\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eDetection range: 0.156-10ng\/mL\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eSensitivity: 0.058ng\/mL\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eUniprot: P08922\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eGene ID: 6098\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eFeatured Series Function: Detects protein (regular version)\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eRecommended\/Predicted Sample Types: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eAssay Time: 3h\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eMethod: Colormetric\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eSpecificity: Reactive with Human ROS1 \/ C-Ros Oncogene 1, Receptor Tyrosine Kinase\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eDetection principle: Double-antibody Sandwich\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eSample Size: 100uL\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eShelf-life: 12 months\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eSpecificity: This assay has high sensitivity and excellent specificity for detection of C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1).\u003cp\u003e\u003c\/p\u003eNo significant cross-reactivity or interference between C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) and analogues was observed.\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) were tested 20 times on one plate, respectively.\u003cp\u003e\u003c\/p\u003eInter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) were tested on 3 different plates, 8 replicates in each plate.\u003cp\u003e\u003c\/p\u003eCV(%) = SD\/meanX100\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\u003cp\u003e\u003c\/p\u003eTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eAssay procedure summary: 1. Prepare all reagents, samples and standards;\u003cp\u003e\u003c\/p\u003e2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;\u003cp\u003e\u003c\/p\u003e3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;\u003cp\u003e\u003c\/p\u003e4. Aspirate and wash 3 times;\u003cp\u003e\u003c\/p\u003e5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\u003cp\u003e\u003c\/p\u003e6. Aspirate and wash 5 times;\u003cp\u003e\u003c\/p\u003e7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;\u003cp\u003e\u003c\/p\u003e8. Add 50µL Stop Solution. Read at 450nm immediately.\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eTest principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eResearch Area: Tumor immunity; \u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eResearch Use Only","brand":"USCN","offers":[{"title":"Default Title","offer_id":45390160953523,"sku":"USEE273Hu-96","price":490.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/USCN.png?v=1774797733","url":"https:\/\/afsbio.com\/products\/elisa-kit-for-c-ros-oncogene-1-receptor-tyrosine-kinase-ros1-usee273hu","provider":"AFSBio Inc.","version":"1.0","type":"link"}