{"product_id":"elisa-kit-for-glucosidase-alpha-acid-gaa-usea177ra","title":"ELISA Kit for Glucosidase Alpha, Acid (GaA) - USEA177Ra","description":"ELISA Kit for Glucosidase Alpha, Acid (GaA)\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eSize: 96T\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eCatalogue Number: USEA177Ra-96\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eCitations, Manuals and MSDS Available upon request.\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eTarget: Glucosidase Alpha, Acid (GaA)\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eAlternative Names: LYAG; Acid Alpha-Glucosidase; Lysosomal Alpha-Glucosidase; Pompe Disease Glycogen Storage Disease Type II; Acid Maltase; Aglucosidase Alfa\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eTarget Species: Rat\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eDetection range: 0.312-20ng\/mL\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eSensitivity: 0.121ng\/mL\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eUniprot: Q6P7A9\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eGene ID: 367562\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eFeatured Series Function: Detects protein (regular version)\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eRecommended\/Predicted Sample Types: Serum, Plasma, Tissue Homogenatesand Other Biological Fluids\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eAssay Time: 3h\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eMethod: Colormetric\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eSpecificity: Reactive with Rat GaA \/ Glucosidase Alpha, Acid\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eDetection principle: Double-antibody Sandwich\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eSample Size: 100uL\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eShelf-life: 12 months\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eSpecificity: This assay has high sensitivity and excellent specificity for detection of Glucosidase Alpha, Acid (GaA).\u003cp\u003e\u003c\/p\u003eNo significant cross-reactivity or interference between Glucosidase Alpha, Acid (GaA) and analogues was observed.\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucosidase Alpha, Acid (GaA) were tested 20 times on one plate, respectively.\u003cp\u003e\u003c\/p\u003eInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucosidase Alpha, Acid (GaA) were tested on 3 different plates, 8 replicates in each plate.\u003cp\u003e\u003c\/p\u003eCV(%) = SD\/meanX100\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\u003cp\u003e\u003c\/p\u003eTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eAssay procedure summary: 1. Prepare all reagents, samples and standards;\u003cp\u003e\u003c\/p\u003e2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;\u003cp\u003e\u003c\/p\u003e3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;\u003cp\u003e\u003c\/p\u003e4. Aspirate and wash 3 times;\u003cp\u003e\u003c\/p\u003e5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\u003cp\u003e\u003c\/p\u003e6. Aspirate and wash 5 times;\u003cp\u003e\u003c\/p\u003e7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;\u003cp\u003e\u003c\/p\u003e8. Add 50µL Stop Solution. Read at 450nm immediately.\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eTest principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucosidase Alpha, Acid (GaA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucosidase Alpha, Acid (GaA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucosidase Alpha, Acid (GaA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucosidase Alpha, Acid (GaA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eResearch Area: Enzyme \u0026amp; Kinase; Metabolic pathway; \u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eResearch Use Only","brand":"USCN","offers":[{"title":"Default Title","offer_id":45390209614003,"sku":"USEA177Ra-96","price":478.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/USCN.png?v=1774797733","url":"https:\/\/afsbio.com\/products\/elisa-kit-for-glucosidase-alpha-acid-gaa-usea177ra","provider":"AFSBio Inc.","version":"1.0","type":"link"}